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The principle and characteristics of the spectrophotometer
作者:www.lab-spectrum.com    发布于:2018-01-17 09:12:19   

Spectrophotometer is the instrument of quantitative and qualitative analysis of substances by spectrophotometry. Spectrophotometry is determined by measuring a test substance in the degree of light absorption in a specific wavelength or a certain wavelength range, the material of qualitative and quantitative analysis. The wavelength range of commonly used: (1) 200 to 400nm UV region, (2), 400 to 760nm of the visible region, (3) 2.5 ~~ 25μm (the wave number is counted as 4000cm <-1> ~ 400cm <-1>) infrared light district. The instrument used an ultraviolet spectrophotometer, a visible spectrophotometer (or colorimeter), an infrared spectrophotometer or atomic absorption spectrophotometer. In order to ensure the precision and accuracy of the measurements, all instruments should be in accordance with national metrological verification or the Appendix provides periodic calibration verification. Monochromatic radiation through the test substance solution, the amount absorbed by the substance is proportional to the concentration of the substance and the liquid layer has a thickness (optical path length), and its relationship to the following formula:
A =-log (I / I) =-Lgt = the KLC formula:
A is absorbance;
I. incident monochromatic light intensity;
I is the transmission of monochromatic light intensity;
T is the transmittance of the material;
k is the absorption coefficient;
L is the optical path of the substances to be analyzed
c is the concentration of a substance

The simple principle of spectrophotometer

Spectrophotometer with a light source can generate a plurality of wavelengths, through a series of spectrophotometric apparatus, thereby generating light of a specific wavelength, the light source through the test sample, after the portion of the light is absorbed, and the absorbance value of the sample is calculated, thereby converted into the concentration of the sample. Proportional to the concentration of the absorbance value of the sample of the sample.

 
 
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